Eksperimental metyhods in biophysics

Enrollment into the program.

Introduction: relation between physics, nanotechnology and biology, overview of physical laws ant typical magnitudes of physical quantities that are relevant on microscopic scales.

Methods of micromanipulation and particle position detection: laser tweezers, optical stretcher, laser scissors, AFM, videomicroscopy, back focal plane interferometry.

Microrheology: passive microrheology, laser and magnetic tweezers based active microrheology, applications in biological systems.

Molecular force spectroscopy: optical tweezers and AFM as force measurement tools on molecular level (DNA, molecular motors, polymeric meshes, proteins).

Microfluidics: fluid flow in micro channels, microfabrication techniques, micropumps and valves, separation nad analytical methods, integrated microfluidics ("Lab on a chip").

Izbrani pregledni in znanstveni članki.
Selected review and research articles.

Objectives:
Student gets familiar with up-to-date experimental technologies that are used in the biophisics reasearch.

Competences:
Knowledge and understanding of principles of operation of experimental methods that are used in biophysics. Knowledge and understanding of experimental planning, performing of measeurements and evaluation of results.

Knowledge and understanding: Understanding of modern equipment and metodologies used in biophysics that enables it’s further development and adaptation to specific requiremnents.

Application: Knowing, understanding and application of physics methods and techniques in biophysics, biology and medicine.

Reflection: Application of physics methods in research and diagnostics of complex biological systems.

Transferable skills:
Ascociation of basic physics principles and theoretical concepts with modern experimental approaches in biophysicsm biology and medicine.

Lectures, exercises, homework, consultations.

Written exam
Oral exam
grading: 5 (fail), 6-10 (pass) (according to the Statute of UL)

MUR, Jaka, KAVČIČ, Blaž, POBERAJ, Igor. Fast and precise Laguerre-Gaussian beam steering with acousto-optic deflectors. Applied optics, ISSN 1559-128X. Tiskana izd., 2013, vol. 52, iss. 26, str. 6506-6511. http://www.opticsinfobase.org/ao/abstract.cfm?uri=ao-52-26-6506, doi:10.1364/AO.52.006506. [COBISS-SI-ID 2608228]

VILFAN, Andrej, BABIČ, Dušan, KAVČIČ, Blaž, KOKOT, Gašper, OSTERMAN, Natan, POBERAJ, Igor, VILFAN, Mojca. Measurement of fluid flow generated by artificial cilia. V: TOONDER, Jaap M. J. de (ur.), ONCK, Patrick R. (ur.). Artificial cilia, (RSC nanoscience & nanotechnology, ISSN 1757-7136). Cambridge: The Royal Society of Chemistry, 2013, str. 244-258. http://pubs.rsc.org/en/content/chapter/bk9781849735971-00244/978-1-84973-597-1. [COBISS-SI-ID 26814759]

KOKOT, Gašper, VILFAN, Mojca, OSTERMAN, Natan, VILFAN, Andrej, KAVČIČ, Blaž, POBERAJ, Igor, BABIČ, Dušan. Measurement of fluid flow generated by artificial cilia. Biomicrofluidics, ISSN 1932-1058, 2011, vol. 5, no. 3, str. 034103-1-034103-9, doi: 10.1063/1.3608139. [COBISS-SI-ID24932135]

OSTERMAN, Natan, POBERAJ, Igor, DOBNIKAR, Jure, FRENKEL, Daan, ZIHERL, Primož, BABIČ, Dušan. Field-induced self-assembly of suspended colloidal membranes. Physical review letters, ISSN 0031-9007. [Print ed.], 2009, vol. 103, str. 228301-1-228301-4, doi:10.1103/PhysRevLett.103.228301. [COBISS-SI-ID 2204516]

KAVČIČ, Blaž, BABIČ, Dušan, OSTERMAN, Natan, PODOBNIK, Boštjan, POBERAJ, Igor. Magnetically actuated microrotors with individual pumping speed and direction control. Applied physics letters, ISSN 0003-6951, 2009, vol. 95, str. 023504-1-023504-3, doi: 10.1063/1.3176969. [COBISS-SI-ID 2184036]